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KMID : 0380319850350000123
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Journal of Korean Research Institute for Better Living
1985 Volume.35 No. 0 p.123 ~ p.128
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The Effect of Morphine and Alcohol with ADH on the Cytochrome P-450, b_5, p-nitroanisole-O-demethylation and AAF-hydroxylation in Rat Liver Microsomes
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Abstract
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Chronic morphine administration to adult male rats has long been known to change hepatic cytochrome P-450 content and its dependent mixed function oxidase activity. More recently, acute treatment of mature male rats with a dose of morphine higher than that used chronically also has been reported to change their hepatic cytochrome P-450
In the present study, the effect of morphine and alcohol by concomitant administration of ADH on the cytochrome P-450, b_5, p-nitroanisole-O-demethylation and AAF-hydroxylation in rat liver microsomes were studied.
The results are as follows;
1. The contensts of cytochrome P-450, and b_5 were increased in the liver microsomes from morphine and ADH-treated rats, whereas they were decreased in liver microsome from alcohol and ADH-treated rats.
2. After administration of morphine and ADH rats, activity p-nitroanisole-O-demethylase, mixed function oxidase system were increased, whereas they were decreased after administration of alcohol and ADH rats.
3. After administration of morphine and ADH rats, the formation of lipid peroxides were decreased, whereas they were increased after administration of alcohol and ADH rats.
No correlation was found between lipid peroxidation and formation of lipid peroxidases, which were induced by morphine and ADH cytochrome P-450 content.
There might be another enzyme catalyzing lipid peroxidation other than cytochrome P-450.
4. The administration of morphine and ADH increased AAF-ring-hydroxylation, compared to ADH group.
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